منابع مشابه
Holliday junction resolvases.
Four-way DNA intermediates, called Holliday junctions (HJs), can form during meiotic and mitotic recombination, and their removal is crucial for chromosome segregation. A group of ubiquitous and highly specialized structure-selective endonucleases catalyze the cleavage of HJs into two disconnected DNA duplexes in a reaction called HJ resolution. These enzymes, called HJ resolvases, have been id...
متن کاملBacterial-type DNA holliday junction resolvases in eukaryotic viruses.
Homologous DNA recombination promotes genetic diversity and the maintenance of genome integrity, yet no enzymes with specificity for the Holliday junction (HJ)-a key DNA recombination intermediate-have been purified and characterized from metazoa or their viruses. Here we identify critical structural elements of RuvC, a bacterial HJ resolvase, in uncharacterized open reading frames from poxviru...
متن کاملThe role of Holliday junction resolvases in the repair of spontaneous and induced DNA damage
DNA double-strand breaks (DSBs) and other lesions occur frequently during cell growth and in meiosis. These are often repaired by homologous recombination (HR). HR may result in the formation of DNA structures called Holliday junctions (HJs), which need to be resolved to allow chromosome segregation. Whereas HJs are present in most HR events in meiosis, it has been proposed that in vegetative c...
متن کاملRecombination: Holliday Junction Resolution and Crossover Formation
The heterodimeric nuclease Mus81-Eme1 has been proposed to be a Holliday junction resolvase and has now been found to be responsible for nearly all meiotic crossovers in fission yeast. The intriguing substrate preference of this enzyme for nicked Holliday junctions opens the possibility that crossover formation may not always involve double Holliday junctions.
متن کاملCommentary All change at Holliday junction
It is probably fair to say that DNA structure holds center stage in many processes of genetic recombination. Understanding the conformation of branched DNA intermediates and their recognition and manipulation by proteins is the key to deducing the underlying mechanisms. In the original Holliday (1) model for homologous genetic recombination, the central intermediate is a four-way junction where...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Perspectives in Biology
سال: 2014
ISSN: 1943-0264
DOI: 10.1101/cshperspect.a023192